Folic Acid Supplementation Study (1999)

The folic acid supplementation study was a double blind placebo-controlled randomized trial. Healthy nonsmoking male subjects were recruited among the staff and students at the University of Kuopio. Exclusion criteria included regular intake of drugs, vitamins or antioxidants, obesity (BMI > 30 kg/m2), and severe diseases. Forty men were randomized to receive either placebo (microcrystalline cellulose) or 300 g folic acid (Foliren®) three times a day for 12 wk. All the subjects provided a written informed consent. The Research Ethics Committee of the University of Kuopio approved the study protocol.

This study was done and samples analyzed in co-operation with Department of Clinical Chemistry, Laboratory of Atherosclerosis Genetics, Tampere University Hospital, and University of Tampere, Medical School, Tampere, Finland (Professor Terho Lehtimäki and researchers Riikka Rontu and Päivi Hämelahti). Oy Verman Ab delivered supplements and partial funded this study.

More information: sari.voutilainen at

Our publications:

Voutilainen S, Rissanen T, Seppänen K, Porkkala-Sarataho E, Kaikkonen J, Tuomainen T-P, Virtanen J, Lehtimäki T, Malin R, Penttilä I, Kaplan GA, Salonen JT. Folic acid increases serum paraoxonase activity: evidence from a double blind oral supplementation trial in men. Current Topics in Nutraceutical Research 2003;1:175-182.

Voutilainen S, Rissanen T, Virtanen J, Lakka TA, Salonen JTS. Low folate intakes are associated with an excess risk of acute coronary events: the Kuopio Ischaemic Heart Disease Risk Factor Study. Circulation 2001;103:2674-2680.


Natural food sorces of folate and.. 


fortified food sources of folate/folic acid.