Technology Platform Services
One of the main aims of Biocenter Kuopio is to strenghten the infrastructure and methodological services, thereby providing technology services to academic and industrial communities mainly in Finland.
In vivo imaging
Kuopio Biomedical Imaging Unit (Kuopio-BIU) provides excellent facilities for small animal in vivo imaging and ex vivo MRI microscopy in different areas of basic and applied research. The imaging unit is located in A.I. Virtanen Institute for Molecular Sciences, UEF, and it consists of three MRI scanners, a SPECT/CT and PET unit for small animal imaging.
- Biomedical Imaging Unit, prof. Olli Gröhn
The Bioinformatics Center provides support and expertise in bioinformatics to researchers. The expert services cover a wide range of tasks including data-analysis, development of computational
methods, consultation and networking. In addition, the center maintains high-end computing servers that UEF researchers can use free of charge.
- Bioinformatics center, Research manager Dr. Wilhelmiina Hämäläinen
Biological Imaging – Electron microscopy & Imaging spectroscopy
SIB Labs is a unit providing services for electron microscopy and imaging spectroscopy. Our equipment and services include modern and high-resolution electron microscopes (FE-TEM and FE-SEM), and related sample processing. Our equipment are very versatile, enabling various imaging modes and elemental analysis for different areas of basic and applied research, as well as for industry. Moreover, we have state-of-the-art microspectroscopes (FTIR and Raman) for chemical imaging and characterization of samples. SIB Labs is a part of Faculty of Science and Forestry, and the above mentioned equipment are located at Kuopio campus.
- SIB Labs, research director Arto Koistinen
Biological Imaging – Light microscopy
Cell and Tissue Imaging Unit provides the instruments to image biological processes from tissues to single cells and subcellular molecular kinetics with both conventional and high-throughput methods. We offer personal user training and support for imaging of fixed or live tissues or cells, including consultation on experimental set up, sample preparation and image processing and analysis. The services include a modern histology laboratory equipped with modern devices and services.
- Cell and Tissue Imaging Unit, Director Kirsi Rilla
Model Organisms - Mammalian model organisms
Phenotyping Center provides research services in neurophenotyping (including studies on the retina) in mice and rats, and cardiovascular phenotyping on mice, rats, rabbits and pigs. Services include behavioral, electrophysiological and histopathological analyses as well as in vivo imaging. Research can be done on a special disease model organism provided by the client or on one of the special disease models provided by BCK group.
- Phenotyping Center, Prof. Heikki Tanila
Proteomics and metabolomics - Metabolomics
The Biocenter Kuopio LC-MS Metabolomics Center focuses on development and use of mass spectrometry based targeted and non-targeted methods in metabolite, drug, and protein analysis. These analyses are related to wide variety of research projects in the areas of medicine, biology, drug development, nutrition, and environmental sciences. The facility has two high-resolution LC-MS instruments (LC-qTOF-MS (Agilent 6540) and LC-Orbitrap-MS (Q-Exactive Focus)), and two LC-QQQ-MS as key instrumentation, related to analysis of wide variety of sample matrixes.
- Metabolomics Center, Prof. Seppo Auriola and Marko Lehtonen
Stem cells and biomaterials
The stem cell core facility of Biocenter Kuopio is a comprehensive provider of human pluripotent stem cell services at A.I. Virtanen Institute, Kuopio campus of the University of Eastern Finland.
- Stem Cell Center, prof. Jari Koistinaho
Structural biology - X-ray, NMR & MS
Structural biology center, located at Department of Chemistry, Joensuu campus, University of Eastern Finland) is based on the use of protein crystallography and high-resolution mass spectrometry.
- Structural biology, prof. Juha Rouvinen
Translational technologies - Drug discovery and chemical biology
Computer-aided drug design (CADD) is a part of modern and rational drug development process. In CADD, various computational methods are applied to speed up identification of new drug candidates, and to improve their properties. The drug design group utilizes both structure-based and ligand-based methods. They use various available and established molecular modeling methods such as docking, similarity searches, 3D-QSAR, pharmacophore modeling, homology modeling and molecular dynamics to identify novel hit compounds or optimize lead structures. Moreover, they study and evaluate the druggability of selected targets and aim to develop high-quality chemical probes useful for pharmacological studies and to identify new therapeutic strategies against cancer, type 2 diabetes and neurogenerative diseases.
Viral gene transfer - Viral gene transfer and cell therapy
National Virus Vector Laboratory provides excellent facilities for production of adenoviral, lentiviral, AAV and baculoviral vectors for in vitro and in vivo work. Laboratory scale and larger scale production methods are available with quality controlled vector backbones. Unit operates one GMP suite and four vector laboratories which are dedicated for each of the vector type. Unit will also give technological support for the use of viral vectors including support for the application of permits required by gene technology law.
- National Virus Vector Laboratory, Academy prof. Seppo Ylä-Herttuala
Genome Editing- Finnish Genome Editing Center (FinGEEC)
Genome Editing- Finnish Genome Editing Center platform provides facilities and equipment for flow cytometric sorting and analysis of cells generated with viral vectors and novel genome editing technologies. Cell preparations can be either cell lines or cells isolated from tissues. Also, instructions and technical support for cell isolation and staining processes for flow cytometry is provided.
- Finnish Genome Editing Center, Dr. Petri Mäkinen
Single Cell Genomics
Single Cell Genomics core provides service in high-throughput single-cell mRNA sequencing. In addition, we provide consultancy in study design and data analysis. Our technology is based on the nanodroplet-based Chromium™ Controller from 10x Genomics that is compatible with Illumina sequencing platforms. The Single Cell Genomics core is located at A.I. Virtanen Institute for Molecular Sciences.
Single Cell Genomics, Associate Professor Minna Kaikkonen-Määttä